Extended Data Fig. 3: Validation of spinal implant with microCT, animal health, and behavior.
From: Long-term optical imaging of the spinal cord in awake behaving mice
a, 3D printed phantom of skull and spinal column. To evaluate impact on microCT scans, a 3D printed spinal chamber (Surgical Guide) is implanted with different cements and metallic screws. b, Horizontal view from microCT scan of phantom in a. Yellow bars: acquisition planes with reconstruction artifacts due to metallic screws; cyan arrows highlight reduced reconstruction of spinal chamber and column. Scale bars, 2 mm. c, Coronal view of scan as in b shows metal screw details and artifact scan lines. Scale bars, 2 mm. d, Coronal sections of the phantom without (left) and with (right) metal screws in the acquisition plane. Scale bar, 2 mm. e, Coronal section from microCT scan (resolution: 20 μm) of a dissected mouse spinal column, placed inside a 3D printed test piece, using the same material (BioMED Clear) as for the 3D printed spinal chamber. Scale bar, 2 mm. f, Off-axis and sagittal views of 3D reconstructed microCT scan as in e. g, Pipeline for 3D reconstruction of microCT scans. h, Coronal view of mouse with 3D printed spinal chamber showing an acquisition plane at the T13 laminectomy location. Scale bar, 2 mm. i, 3D reconstruction of the mouse in Fig. 1h-j and h with bone (gray), spinal chamber (blue), and glass coverslip (red). Inset: magnified view highlights the T13 laminectomy and spinal chamber. j, Change in weight of an additional cohort of individual animals after chamber implant. Two mice, ‘2’ and ‘3’, are replotted from Fig. 1l. k, Model error (sum of score map cross-entropy and body part location L1-distance losses) as a function of DeepLabCut iterations for model trained (600,000 iterations) using data from 3 mice in an open field. l, Mean (per animal) latency to fall in all three trials on an accelerating rotarod, comparing naĂ¯ve (n = 14) and different post-surgery times (n = 12/8/8, 2, 10, 5, 5, 5, 5). Error bars are mean ± SD. Two-way ANOVA including all trials followed by one-way ANOVA with Dunnett post-hoc per trial (one star, P < 0.05).
