Extended Data Fig. 4: Schematic of the data processing procedure.
From: Elemental mapping in single-particle reconstructions by reconstructed electron energy-loss analysis
Spectral images were processed with tools from the Hyperspy framework or the software RELION, as indicated by green or blue arrows, respectively. For the reference workflow, elastic bright-field images were extracted from the spectral images. Suitable particles were picked and extracted from these, subjected to 2D classification for cleaning and then pre-aligned with a 3D classification job and further refined in a 3D Refine job. For resolution estimation, the results were further subjected to postprocessing. For the full spectral workflow, micrographs were extracted from the spectral images at all energies, particles were reextracted from these micrographs using the previously refined particle positions and then reconstructed for each energy on the basis of the poses determined in the reference workflow. Spectra of specific voxels or summed spectra of subvolumes were then analysed from these reconstructions in Hyperspy.
