Extended Data Fig. 5: Maps reconstructed in a modified version of RELION show different intensities of corner artefacts depending on padding factors. | Nature Methods

Extended Data Fig. 5: Maps reconstructed in a modified version of RELION show different intensities of corner artefacts depending on padding factors.

From: Elemental mapping in single-particle reconstructions by reconstructed electron energy-loss analysis

Extended Data Fig. 5

The reconstruction at 284 eV is shown for three different padding factors (Fourier volume subsampling) with Gaussian filtering with a standard deviation of 7.3 Å applied for better visualisation. No padding yields a strong artefact that dominates the reconstruction. This is reduced by padding. With a padding factor of 2, the artefact is only visible in the corners of the box, while with a padding factor of 8, the artefact does not overlap with the reconstruction at relevant thresholds. The scale bar corresponds to 10 nm and applies to all volumes.

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