Extended Data Fig. 7: Selected spectra and reconstructions of reconstructed EELS data of WH.

(a) As for RyR1, the spectrum of the sum of all intensities in the EELS reconstructions shows elemental edges for the most abundant elements: carbon, nitrogen, and oxygen. The asterisks mark regions where the spectra show dips due to double-sized pixels at the tile boundaries of the detector, which are not correctly accounted for by the detector’s flatfields. (b) For the region between 118 and 240 eV a background subtraction was performed, based on a fit of the spectrum between 121 eV and 127 eV. The subtracted spectrum shows additional edges for phosphorus and chlorine. (c-e) Gaussian-filtered reconstructions for three 4.6 eV-regions of the spectrum are shown together with PDB-5M3L ref. ²⁵. Before the carbon edge (c), the reconstruction shows uniform distribution of intensity between the protein and the solvent. At the carbon edge (d), the reconstruction shows colocalization with the protein, and at the oxygen edge (e), the reconstruction shows colocalization with the solvent. The reconstructions reflect the known local distributions of carbon and oxygen. The scalebar near panel c corresponds to 10 nm and applies to panels c-e.