Fig. 6: Evaluating the resolution of EELS reconstructions and the feasibility and quality of localized background subtraction.
From: Elemental mapping in single-particle reconstructions by reconstructed electron energy-loss analysis
a, The resolution of the energy-loss reconstruction at the carbon edge is estimated by reconstructing and summing halfmaps in the edge region and calculating their FSC, giving a resolution of 40 Å. b, A spectrum of a single voxel of raw reconstructions, a spectrum of the same voxel after applying Gaussian filtering with a standard deviation (sDev) of 2 voxels to the volumes and background fits to the latter before the carbon and before the nitrogen edge. c, The carbon reconstruction looks similar before and after background subtraction. d, The nitrogen reconstruction after background subtraction shows less intensity in the region of the micelle, as the background subtraction effectively removes the signal due to the long tail of the carbon edge below the nitrogen edge signal. Comparing the background-subtracted maps for carbon and nitrogen, the differences in the micelle region are reflective of the lower N:C ratio in the micelle than in the protein region. The scale bar near c corresponds to 10 nm and applies to c–e.
