Extended Data Fig. 2: Schematic of the procedure for automated collection of spectral images.
From: Elemental mapping in single-particle reconstructions by reconstructed electron energy-loss analysis
using the microscope control software SerialEM in communication with the filter software Panta Rhei via its RPC interface. After preparation steps, as indicated, automated acquisition is started. For every acquisition position, the stage and the microscope optics are set for the acquisition by SerialEM, then an external RPC script is called, which triggers acquisition and saving of a spectral image in Panta Rhei.
