Extended Data Fig. 5: epegRNA and endogenous target features vary in their impact on phenotype.

a, Growth phenotypes for stop epegRNAs (top two per gene) from StopPR screen sampled from day 14 post transduction, binned by indicated features. Feature categories and analyzed epegRNAs comprise those used for effect size analysis in Fig. 4a. For features with two categories, a two-sided, two-sample t-test was performed. For features with three categories, a one-way ANOVA followed by a two-sided Tukey post-hoc was performed. Resulting p-values indicated and number of epegRNAs analyzed for each feature category denoted as n. F or t statistics are as follows (left to right, top to bottom): 115.9, 29.2, -7.73, -4.91, 5.27, 11.4, 2.61, and -2.42. Median and interquartile range (IQR) of the full set of epegRNAs used in this analysis per group are indicated. Whiskers extend 1.5*IQR past the upper and lower quartiles. b, Average growth phenotypes for stop epegRNAs specifying individual substitution types targeted in StopPR with both sense (blue) and antisense (red) protospacers. Data from cells sampled from day 14 post transduction of library. Numbers of stop epegRNAs denoted as n and p-values from two-sided, two-sample t-test indicated. c, Schematic of sense and antisense protospacers relative to gene.