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Integrative spatial protein profiling with multi-omics

Nature Methods volume 21, pages 2223–2225 (2024)Cite this article

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Spatial proteomics holds the potential to transform the study of proteins in situ in complex tissues, but it needs to be integrated with other layers of omics data to gain a holistic view of cellular function, heterogeneity and interactions, and the underlying mechanisms of these processes. I highlight current challenges and emerging opportunities for multi-omic spatial protein profiling to advance basic research and translational applications.

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Fig. 1: Understanding spatially resolved proteomics in the context of multi-omics.

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Acknowledgements

R.F. is supported by Packard Fellowship for Science and Engineering, Yale Stem Cell Center Chen Innovation Award, and grants from the US National Institutes of Health (U54AG076043, U54AG079759, UG3CA257393, UH3CA257393, R01CA245313, RF1MH128876, U54CA274509, U54CA268083, and U01CA294514).

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Authors and Affiliations

  1. Department of Biomedical Engineering, Yale University, New Haven, CT, USA

    Rong Fan

  2. Department of Pathology, Yale University School of Medicine, New Haven, CT, USA

    Rong Fan

  3. Yale Cancer Center, Yale University School of Medicine, New Haven, CT, USA

    Rong Fan

  4. Yale Stem Cell Center, Yale University School of Medicine, New Haven, CT, USA

    Rong Fan

  5. Yale Center for Aging Biology Research (Y-Age), Yale University School of Medicine, New Haven, CT, USA

    Rong Fan

  6. Human and Translational Immunology, Yale University School of Medicine, New Haven, CT, USA

    Rong Fan

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  1. Rong Fan
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Correspondence to Rong Fan.

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Competing interests

R.F. is co-founder of and scientific advisor to IsoPlexis, Singleron Biotechnologies, and AtlasXomics.

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Fan, R. Integrative spatial protein profiling with multi-omics. Nat Methods 21, 2223–2225 (2024). https://doi.org/10.1038/s41592-024-02533-x

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