We provide scalable new tools, which include mosaic models and downstream multiplexing, for longitudinal single-cell profiling of brain organoids derived from multiple individuals. In addition, we benchmark computational methods for cell identity deconvolution and provide guidelines on how to use each multiplexing method for charting neurodevelopmental trajectories.
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This is a summary of: Caporale, N. et al. Multiplexing cortical brain organoids for the longitudinal dissection of developmental traits at single-cell resolution. Nat. Methods https://doi.org/10.1038/s41592-024-02555-5 (2024).
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Multiplexing strategies to scale up brain organoid modeling. Nat Methods 22, 235–236 (2025). https://doi.org/10.1038/s41592-024-02556-4
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DOI: https://doi.org/10.1038/s41592-024-02556-4
