Extended Data Fig. 1: PCA & UMAP of A549 cells with relative abundancies of selected cell-cycle specific proteins color coded. | Nature Methods

Extended Data Fig. 1: PCA & UMAP of A549 cells with relative abundancies of selected cell-cycle specific proteins color coded.

From: Challenging the Astral mass analyzer to quantify up to 5,300 proteins per single cell at unseen accuracy to uncover cellular heterogeneity

Extended Data Fig. 1

a–n, Individual A549 cells were prepared using the One-Pot workflow. Digested cells were analyzed at 50 SPD using the previously optimized settings for LC and MS on the Orbitrap Astral MS. The color scale bars depict the log2-transformed protein abundance. PCA (a-g) and UMAP (h-n) are based on protein quantities with each dot representing a cell and colors reflecting the relative abundance of the protein given.

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