Extended Data Fig. 10: Identification of super-photostable photoblued PF610 derived from the photoblueing of Sulfo-Cy7.
From: Super-photostable organic dye for long-term live-cell single-protein imaging
a. In vitro photobleaching lifetime analysis of PF610 and AF647. The upper panel displays bulk fluorescence images of PF610 in the far-red channel (654–870 nm). Scale bar = 20 μm. The lower panel shows the averaged fluorescence intensity profiles fitted with exponential decay curves of PF610 (red line and dots) and AF647 (blue line and dots). b. The single-molecule intensity profile of PF610. The upper panel features single-molecule images of PF610 in the far-red channel (654–870 nm). Scale bar = 1 μm. The lower panel presents the fluorescence intensity profile of single PF610 molecules over time. c. HPLC chromatography comparison before (dash) and after (solid) the Sulfo-Cy7 photoblueing reaction. Different colors in the chromatogram indicate different absorption wavelengths, ranging from 500–630 nm, of the corresponding compounds. d. Excitation (yellow line) and emission spectrum (pink line) of PF610 measured in distilled water.
