Extended Data Fig. 3: Decomposition outputs of Spotiphy and iscRNA data construction.
From: Spotiphy enables single-cell spatial whole transcriptomics across an entire section
a, Workflow summary for ST data decomposition and iscRNA data construction. b, c, UMAP projection of 33,819 cells from iscRNA data produced by applying Spotiphy to mouse brain Visium data. Clusters are labeled according to cell type (b) or sample-of-origin (c). d, UMAP projection of 7,083 astrocytes extracted from iscRNA data produced by applying Spotiphy to mouse brain Visium data. Clusters are labeled according to the original clusters. e, Bar plots of astrocyte subtype proportions annotated from Fig. 3a for both WT and AD mouse samples. f, Diagram for sagittal section of mouse brain labeled with different topographic regions. CTX: cerebral cortex; HPF: hippocampus; FT: fiber tracts; TH: thalamus; HY: hypothalamus; STR: striatum. Allen Mouse Brain Sagittal Atlas, https://mouse.brain-map.org/static/atlas. Scale bars: 500 μm. g, Result of applying NetBID2 GSEA analysis to iscRNA data of astrocytes in CTX vs. TH.
