Fig. 1: Overview of the SG-NEx datasets and processing pipeline.

a, Seven human cell lines were sequenced with multiple replicates using different RNA-seq protocols. Short-read cDNA was sequenced with 150-bp paired-end reads. hES cells, human embryonic stem cells. Icons from Noun Project under a Creative Commons license CC BY 3.0: colon, Mungang Kim; leukocytes, ProSymbols; liver, Prettycons; lung, Mahmure Alp; breast, Karina; ovary, Amethyst Studio; hES cells, DailyPM. b, Number of sequencing runs generated for each SG-NEx core cell line. c, Number of sequencing runs for each of the RNA-seq technologies. d, Illustration of the nf-core Nextflow pipeline (nanoseq) for streamlined processing of Nanopore long-read RNA-seq data.