Extended Data Fig. 5: DNA model training results. | Nature Methods

Extended Data Fig. 5: DNA model training results.

From: Uncalled4 improves nanopore DNA and RNA modification detection via fast and accurate signal alignment

Extended Data Fig. 5

(a) Current levels from Uncalled4 and ONT r9.4.1 6-mer DNA models. (b) Current levels from Uncalled4 and ONT r10.4.1 400 bps 9-mer DNA models. Inset displays sequence logo for k-mers with more than 0.5 normalized units of difference between the models (indicated on main plot by dashed line). (c) Current distributions for k-mers with each base fixed at the 6th and 5-th positions for r10.4.1 models, including both 400 bps and 260 bps ONT models. Most distributions are unimodal, except for ONT 400 bps which has outliers caused by ‘TVTT’ k-mers. (d) Comparison between Uncalled4’s r10.4.1 400 bps model and ONT’s 260 bps model, which lacks the outliers seen in ONT’s 400 bps model.

Back to article page