Extended Data Fig. 10: Cell line m6Anet analysis. | Nature Methods

Extended Data Fig. 10: Cell line m6Anet analysis.

From: Uncalled4 improves nanopore DNA and RNA modification detection via fast and accurate signal alignment

Extended Data Fig. 10

(a) Distance from annotated stop codon for transcript-level m6A sites found by Uncalled4 (purple) and Nanopolish (green) with m6Anet at matched 85% precision. (b) Metagene plot of the same m6A sites, with the distribution of reference DRACH sites (gray) and DRACH sites covered by the nanopore reads (orange). (c) Gene-level m6A counts by the number of cell lines they were found in, divided into putative ‘true positives’ (TP, in the m6A-Atlas) and putative ‘false positives’ (FP, missing from the m6A-Atlas).

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