Extended Data Fig. 1: Comparisons between RNA pore model per-k-mer current means.

(a) Comparison between the five central bases of ONT’s 9-mer RNA004 model and an Uncalled4-trained RNA002 5-mer model. (b) Uncalled4-trained RNA002 model compared with and ONT ‘rna_r9.4_180mv_70bps’ model, which is the default model that Uncalled4 and Nanopolish use for RNA001 or RNA002. (c) Boxplots showing distribution of differences between the mean current of signal aligned to the HEK293T reference and the current predicted by the k-mer model. Boxes span the first and third quartiles with the median indicated by the horizontal line, and whiskers extend to 1.5 times the interquartile range.