Extended Data Fig. 4: Modification signal trackplots. | Nature Methods

Extended Data Fig. 4: Modification signal trackplots.

From: Uncalled4 improves nanopore DNA and RNA modification detection via fast and accurate signal alignment

Extended Data Fig. 4

Trackplots displaying per-read-k-mer mean current levels for Uncalled4 and Nanopolish in-vitro transcribed and native E. coli ribosomal RNA sequenced with ONT RNA002. An O6-methylguanine site is present in the native dataset at position 526, causing a drop in current. White cells indicate masked positions, where Uncalled4 performs no masking in this dataset because there were no large insertions or deletions, while Nanopolish masks many positions particularly around the modification site.

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