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Pooled CRISPR screening with optical sequencing reveals regulators of 3D chromatin organization

Nature Methods volume 22, pages 901–903 (2025)Cite this article

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The Perturb-Tracing approach represents a significant step forward in our ability to perform pooled loss-of-function perturbations to identify regulators of 3D genome organization across multiple length scales.

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Fig. 1: Schematics of pooled CRISPR screening strategies.

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Acknowledgements

I thank all the members of my lab for fruitful discussions. This article and the research in the Adli lab are supported by the following NIH grants: 1R01CA267544 (PI: Adli), 1UM1HG012649 (PI: Adli), and U54CA268084 (PI: Backman).

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  1. Feinberg School of Medicine at Northwestern University, Robert H. Lurie Comprehensive Cancer Center, Department of Obstetrics and Gynecology, Chicago, IL, USA

    Mazhar Adli

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  1. Mazhar Adli
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Correspondence to Mazhar Adli.

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The author declares no competing interests.

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Adli, M. Pooled CRISPR screening with optical sequencing reveals regulators of 3D chromatin organization. Nat Methods 22, 901–903 (2025). https://doi.org/10.1038/s41592-025-02633-2

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