Extended Data Fig. 2: Entropy similarity distribution between denoised and raw NIST 23 Orbitrap MS/MS spectra. | Nature Methods

Extended Data Fig. 2: Entropy similarity distribution between denoised and raw NIST 23 Orbitrap MS/MS spectra.

From: Denoising Search doubles the number of metabolite and exposome annotations in human plasma using an Orbitrap Astral mass spectrometer

Extended Data Fig. 2

This histogram illustrates the distribution of entropy similarities between 10,000 randomly sampled denoised NIST 23 Orbitrap MS/MS spectra, against their corresponding raw spectra. The mode, mean and median spectral entropy similarity are all above 0.99. This means spectra that have already high quality (like NIST23 spectra) are not significantly affected by electronic and chemical denoising.

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