Extended Data Fig. 3: Tradeoffs between deep-targeted sequencing and modest whole-genome sequencing for ctDNA detection. | Nature Methods

Extended Data Fig. 3: Tradeoffs between deep-targeted sequencing and modest whole-genome sequencing for ctDNA detection.

From: Error-corrected flow-based sequencing at whole-genome scale and its application to circulating cell-free DNA profiling

Extended Data Fig. 3

A Mutational burden (number of SNVs) of 22 cancer types retrieved from the Pan Cancer Analysis of Whole Genomes consortium. The numbers along the x-axis represent the number of tumors analyzed per cancer type. B Median ctDNA detection opportunities using a whole-genome approach with 10x sequencing coverage, a 10-target panel at 10,000x coverage and a 1-target panel at 10,000x coverage. The pink shaded area represents tumor types for which targeting only a few sites may offer benefit over whole-genome sequencing. The blue shaded area represents tumor types for which a whole-genome approach will offer more opportunities to detect ctDNA over targeted panels. The lower and upper ends of the boxplots in (A) represent the 25th and 75th percentiles of the data, respectively, and the horizontal lines represent the median. The whiskers represent at most 1.5 times the IQR.

Back to article page