Extended Data Fig. 6: Comparison of detected UV-derived mutations using duplex, single-strand and UMI-agnostic denoising methods. | Nature Methods

Extended Data Fig. 6: Comparison of detected UV-derived mutations using duplex, single-strand and UMI-agnostic denoising methods.

From: Error-corrected flow-based sequencing at whole-genome scale and its application to circulating cell-free DNA profiling

Extended Data Fig. 6

A Cosine similarities by cancer stage at baseline timepoints (pre-treatment or pre-surgery) for UV and CH-associated signatures. B Comparison of duplex, single-strand and UMI-agnostic denoising methods to detect melanoma-associated variants using a single-read variant calling pipeline for pre-treatment plasma samples from melanoma patients (top) and cancer-free controls (bottom). P-values were measured using a two-sided Wilcoxon test. For all boxplots, the lower and upper ends of boxes represent the 25th and 75th percentiles of the data, respectively, and the horizontal lines represent the median. The whiskers represent at most 1.5 times the IQR.

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