Extended Data Fig. 9: Time course of transsynaptic tracing from mPFC to striatum.
From: ATLAS: a rationally designed anterograde transsynaptic tracer
a. Coronal section from PFC of a mouse injected with AAV8-ATLASsnCre and AAV8-BACE-HA in PFC, and AAV8-DIO-GFP in Str, and incubated for four days. b. Coronal section from the same mouse as in a. c. Coronal section from PFC of an experiment similar to that in a. incubated for 7 days. d. Coronal section of Str from the same mouse as in c. e. Coronal section from PFC of an experiment similar to that in a. and c. incubated for 14 days. f. Coronal section of Str from the same mouse as in e. g. Coronal section from PFC of an experiment similar to that in a., c., and e. incubated for 21 days. h. Coronal section of striatum from the same mouse as in g. i. The number of cells labeled in the PFC between 4 days and 21 days. j. The total intensity of GFP labeling in Str between 4 and 21 days. k. The number of cells labeled with GFP in Str between 4 and 21 days. Each data point represents a biological replicate in a different mouse. Scale bars 100 μm.
