Fig. 3: ATLAS mediates transsynaptic tracing in vivo.
From: ATLAS: a rationally designed anterograde transsynaptic tracer
a, Schematic depicting injections of AAV8-ATLASCre into the mPFC (cyan) and AAV8-DIO-mCherry into the Str (red) to trace the unidirectional projection from the mPFC to Str. b, ALFA-tag (At) staining of ATLASCre in presynaptic cells in mPFC in a wild-type (WT) mouse. c, mCherry staining of postsynaptic cells in Str in the same brain as b. d, Control brain injected only with AAV8-DIO-mCherry in Str. e, Schematic depicting injections of AAV8-DIO-ATLASFLP and Lenti-CamkII-Cre into the primary visual cortex (V1, cyan) and AAV8-fDIO-mCherry into the SC (red) to trace the unidirectional projection from V1 to SC. f, At staining of ATLASFLP in presynaptic cells in V1 in a wild-type mouse. g, mCherry staining of postsynaptic cells in SC in the same brain as f. h, Control brain injected only with AAV8-fDIO-mCherry in SC. i, Schematic depicting injections of AAV8-DIO-ATLASFLP into the dLGN (cyan) and AAV8-fDIO-mCherry into V1 (red) to trace the projection from dLGN to V1 in a VIPR2-Cre mouse. j, At staining of ATLASFLP in presynaptic cells in dLGN. k, mCherry staining of postsynaptic cells in the V1 in the same brain as j. l, Control brain injected with only AAV8-fDIO-mCherry in V1. Scale bars, 100 μm.
