Fig. 1: ‘Digitalized’ organoids principle. | Nature Methods

Fig. 1: ‘Digitalized’ organoids principle.

From: Digitalized organoids: integrated pipeline for high-speed 3D analysis of organoid structures using multilevel segmentation and cellular topology

Fig. 1

a, Schematic depicting the pipeline. Top, Organoids were imaged using 3D fluorescence microscopy. Middle, The complete 3D digitalization of organoids consists of three layers of segmentation. Bottom, The resulting data are understandable through data mining. b, Nuclei and cell segmentation applied on a primary PDAC organoid. The white arrows indicate low-intensity nuclei.

Back to article page