Extended Data Fig. 6: A case study of neuron projection research using NeuroXiv.

a, this study includes 417 VPM neurons from two sources. Overall, VPM neurons project through the CP brain region, bifurcating at the boundary of the CP region to target cortical brain areas. Based on combinations of target brain regions, eight subtypes of projections are identified. Subtype P8 (characterized by neurons projecting to VISp) was excluded from subsequent analyses. b, differences in projection strength among various projection types in target brain regions and cortical layers are visualized, with projection strength determined by axonal length. c, VPM neurons exhibit projection selectivity across cortical layers. For instance, neurons projecting solely to the SSp-n region form clusters while skipping L5, whereas neurons projecting solely to the SSp-m or SSp-bfd regions form clusters while skipping L4. d and e, differences in soma distribution and morphological characteristics among various VPM projection subtypes are analyzed. In this figure, box edges in box plots show 25th and 75th percentiles, the centre line shows the 50th percentile, and bars show 1.5× the interquartile range (75th percentile – 25th percentile).