Fig. 1: Comparison of sCMOS, EMCCD and QIS cameras.
From: A telescopic microscope equipped with a quanta image sensor for live-cell bioluminescence imaging
a–c, Three-dimensional plots of the same fluorescent slide measured under identical conditions (Supplementary Fig. 1a), but with the sCMOS (a), EMCCD (b) and QIS (c) cameras. ND filters were used to normalize the photon flux per pixel. Exposure time, 2 s. d–f, Fluorescence images of MEFs labeled with quantum dots acquired by the sCMOS (d), EMCCD (e) and QIS (f) cameras, under identical conditions and adjusted for photon flux per pixel. Exposure time, 10 s. Effective pixel size of a and d, 812.5 nm. Effective pixel size of b and e, 2,000 nm. Effective pixel size of c and f, 137.5 nm. ND filters used: sCMOS, ND1.5 (NE10B-A and NE05B-A, Thorlabs); EMCCD, ND 2.4 (NE20B-A and NE04B-A, Thorlabs). No ND filters were used for the QIS. g, Sensor size comparison of the EMCCD and QIS when using the same optical setup to image the same sample of bioluminescent cells (same sample as in Extended Data Fig. 4). Exposure time, 100 s. Representative results are shown from two to three independent experiments.
