Extended Data Fig. 6: Imaging Intracellular and Extracellular Vesicles on LV200/EMCCD with 40× Objective Lens. | Nature Methods

Extended Data Fig. 6: Imaging Intracellular and Extracellular Vesicles on LV200/EMCCD with 40× Objective Lens.

From: A telescopic microscope equipped with a quanta image sensor for live-cell bioluminescence imaging

Extended Data Fig. 6

a, b Imaging intracellular (a) and extracellular (b) vesicles from NLuc-CD63 cells with different contrast settings for the same image under 0.3 s exposure time. c, d Imaging intracellular (c) and extracellular (d) vesicles with different contrast settings for the same image under 2 s exposure time. EV trails between cells are visible with the 40× objective and longer integration time. Substrate: Nano-Glo Live Cell Substrate. Effective pixel size: 2000 nm. Representative results are shown from five independent experiments.

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