Extended Data Fig. 9: Toxicity and Bleaching Comparison of Fluorescence and Bioluminescence.
From: A telescopic microscope equipped with a quanta image sensor for live-cell bioluminescence imaging
a–c, Time-lapse images of the fluorescence of Gamillus-CD63 expressed in MEFs. embryonic. The same image contrast settings have been applied to each image. Exposure time: 3 ms. d, Transmission brightfield image shows that cell morphology changed significantly after 60 minutes of measurement. e–g, Time-lapse images show the bioluminescence of NLuc-CD63 MEFs. Exposure time: 0.3 s. Substrate: Nano-Glo Live Cell Substrate. h, Transmission brightfield image shows that cell morphology remained essentially unchanged after 60 min of measurement. Effective pixel size: 423 nm. Representative results are shown from three independent experiments.
