Fig. 2: Benchmarking the QIScope against the LV200/EMCCD.
From: A telescopic microscope equipped with a quanta image sensor for live-cell bioluminescence imaging
a,d, Schematic of LV200/EMCCD with a 100× oil-immersion objective lens (a) and QIScope with a 40× oil-immersion objective lens, a 20× objective lens as the tube lens and a Keplerian telescope in between (d). For epifluorescence experiments, an emission filter was placed within the telescope. b,c, Comparison of the LV200/EMCCD (b) and QIScope (c) FOVs gives 514 μm and 1,835 μm, respectively. e,f, The resolution test target imaged at 600 and 700 parallel lines per mm (pl mm−1) by the LV200/EMCCD (e) and QIScope (f), respectively. g, Comparison of the MTFs for the two microscopes. Polynomial fits to data points are guides for the eye. The resolution limit of the LV200/EMCCD is 680 pl mm−1 and of the QIScope is 1,200 pl mm−1. h,i, Intensity profiles with Lorentzian fits measured from a fluorescent slide under identical excitation conditions for LV200/EMCCD (h; SNR = 17.5) and QIScope (i; SNR = 22.9). Exposure time, 10 s. Effective pixel size of LV200/EMCCD, 800 nm. Effective pixel size of QIScope, 423 nm.
