Fig. 3: Imaging of EV bioluminescence by the LV200/EMCCD and QIScope.
From: A telescopic microscope equipped with a quanta image sensor for live-cell bioluminescence imaging
a,e, Bioluminescence images at low contrast of MEFs expressing NLuc–CD63 acquired under identical conditions by the LV200/EMCCD (a) and QIScope (e). Exposure time, 2 s. Substrate, Nano-Glo Live Cell Substrate. b,f, Images of the gray dashed boxed area in a and e under high-contrast settings. c,g Denoised versions of b and f. d,h, The intensity profiles of the orange lines in a and e are plotted along with the dynamic range. Effective pixel size of LV200/EMCCD, 800 nm. Effective pixel size of QIScope, 423 nm. Representative results are shown from three independent experiments.
