Fig. 4: Multimodal imaging on the QIScope: bioluminescence and epifluorescence.
From: A telescopic microscope equipped with a quanta image sensor for live-cell bioluminescence imaging
a, The bioluminescence of NLuc–CD63 (turquoise) and the fluorescence of MitoTracker Red CMXRos dye (purple) of the same MEFs imaged sequentially on the QIScope. b,c, Isolated bioluminescence signal from the white square in a at low (b) and high (c) image contrast. EVs were observed at high contrast in bioluminescence (arrow). Exposure time, 2 s. Substrate, Nano-Glo Live Cell Substrate. d,e, Isolated fluorescence signal from the same white square in a at low (d) and high (e) image contrast. No EVs were observed by fluorescence (arrow). Regularly spaced dots in c and e correspond to imprinted grids on the chamber surface. Exposure time, 0.5 s. Effective pixel size, 423 nm. Representative results are shown from five independent experiments.
