Extended Data Fig. 2: Properties of the 40×/20× Two-objective Microscope.
From: A telescopic microscope equipped with a quanta image sensor for live-cell bioluminescence imaging
a, Schematic of the 40×/20× two-objective microscope. L1: Lens 1, L2: Lens 2, S: Sample, OL: Objective lens, BS: Beamsplitter (BSN10R, Thorlabs), F1: 500 nm Longpass Filter, TL: Tube lens. Distance between OL and TL: ~30 mm. Representative results are shown from two independent experiments. b, Transmission illumination image of a resolution target shows strongly restricted field of view (FOV = 325 μm). c, d, Intensity profiles measured from a fluorescent slide under the identical setup except with the tube lens exchanged: LV200 tube lens (c) and 20× objective lens as tube lens (d).
