Extended Data Fig. 2: Polysome ReLiC screen for regulators of mRNA translation.
From: Decoding post-transcriptional regulatory networks by RNA-linked CRISPR screening in human cells
a. Correlation between replicates. Points represent individual sgRNAs in the ReLiC library. Polysome to monosome ratios are median-centered across sgRNAs in the library. r refers to Pearson correlation coefficient. b. Gene ontology analysis of perturbations that decrease heavy polysome to monosome ratio. Gene ontology analysis performed using GOrilla [@Eden2009] and a subset of enriched terms representative of specific gene classes are shown. c. Change in polysome to supernatant ratio for ribosomal protein and ribosome biogenesis genes. Closed circles correspond to gene hits (FDR < 0.05 with 3 or more concordant sgRNAs). d. Comparison of heavy polysome to monosome and heavy polysome to supernatant ratios for selected translation-related factors. e. Polysome profiles of cell lines depleted of screen hits Profiles are normalized by 80S peak height. P/M indicates ratio of area under the curve for polysome fractions to monosome fractions. f. Comparison of heavy polysome to monosome ratio with growth fitness measured by mRNA and genomic DNA barcode seqencing. g. Comparison of heavy polysome to monosome ratio with growth fitness measured by genomic DNA barcode sequencing for gene knockouts in specific groups. Points correspond to genes targeted in the ReLiC-RBP library. Shaded areas correspond to 95% confidence intervals for a linear fit of polysome to monosome ratio to growth fitness within each gene group.
