Extended Data Fig. 3: Isoform-specific splicing screen using ReLiC.
From: Decoding post-transcriptional regulatory networks by RNA-linked CRISPR screening in human cells
a. Number of gene hits that increase the level of the indicated reporter isoform on various days after Cas9 induction. b. Correlation between barcode sets. For each sgRNA, individual linked barcodes were randomly partitioned into two sets, as in Extended Data Fig. 1f. Each point represents a unique gene that was classified as a hit either with barcode Set A or barcode set B. r refers to Pearson correlation coefficient between barcode sets. c. Correlation between relative levels of different mRNA isoforms. Values represent Pearson correlation coefficients for pairwise comparison between the two barcode sets in b. d. Depletion of genomic DNA barcodes corresponding to SF3b complex subunits after Cas9 induction.
