Extended Data Fig. 4: Dissecting mRNA quality control using ReLiC.
From: Decoding post-transcriptional regulatory networks by RNA-linked CRISPR screening in human cells
a. Validation ofβ-globin NMD reporters. Vertical axis represents − ΔΔCt value of indicated reporter mRNA relative to mCherry-Puro control mRNA. Error bars denote standard error of qPCR across 3 technical replicates. b. Gene ontology analysis of perturbations that increase PTC reporter mRNA levels. c. Volcano plot of reporter mRNA levels with dual barcode screen. Each point corresponds to a gene targeted by the ReLiC library. Marker shape and color denotes one of highlighted gene groups. Genes with FDR < 0.05 and belonging to one of the highlighted groups are listed in the legend. Vertical axis indicates P-values from a permutation test as calculated by MAGeCK. d. PTC reporter mRNA levels for individual translation initiation complex subunits. Error bars denote standard deviation across all detected sgRNAs for that gene. Vertical axis indicates P-values from a permutation test as calculated by MAGeCK; ***: P < 0.001, **: 0.001 < P < 0.01, *: 0.01 < P < 0.05; all other genes have P > 0.05. e. Growth fitness after depletion of translation initiation complex subunits.
