Extended Data Fig. 9: Comparation of data quality of SARS-CoV-2 (the Alpha strain) sequencing achieved with our r2r-fl prototype and the NovaSeq 6000 flow cell.
From: Fast, cost-effective and flexible DNA sequencing by roll-to-roll fluidics
a, Heatmap of Q30 for the whole biochip, where each green dot indicates a field of view (FOV) of microscope with high data quality (Q30 > 80% before filtration), and the red regions around the edges indicate poor quality for base calling algorithm. A selected region used for image registration is enclosed in a dash-line circle. b, A zoomed-in fluorescent image from A channel, where each bright spot indicates a DNB that adding Adenine nucleotides in current cycle of bioreaction. Arrows indicating track lines that with less DNBs divides different units of square region and help registration and alignment of images from different cycles. c, Q30 heatmap of the flow cell (Novaseq 6000). d-h, Comparison of the fluorescent intensity, lag, run on, base distribution, filtered average Q30 for the first 50 cycles for the flow cell and r2r-fl cell are shown in (d), (e), (f), (g), and (h), respectively. Flow cell data sourced from basespace.illumina.com.
