Extended Data Fig. 1: Structure, flows, and meshing of pressure-driven microfluidic flow cells.
From: Fast, cost-effective and flexible DNA sequencing by roll-to-roll fluidics
a, Photos of flow cells by Illumina: the high-throughput four-channel S4 flow cell for NovaSeq 6000, the medium-throughput four-channel flow cell for NextSeq 550, and the small-throughput single-channel flow cell for MiSeq. BGI’s fastest flow cell, DNBSEQ-T7 is also shown (cover glass breakage occurs above 90 kPa at the zones indicated with white arrows). Images courtesy of Illumina, Inc and MGI Tech Co. b, Structure (not to scale) of one lane for a typical flow cell (DNBSEQ-G400), its side view (c) and frontal view (d) of the flow cell with inlet and outlet tubing. The inset shows the gap height. e, The velocity field for the Poiseuille flow in the biochip chamber, which is approaching 0 when it is close to the walls. f-h, Meshing of the pressure-driven microfluidic flow cell for computational fluid dynamics (CFD) calculations. Top view (f) and side view (g) of the mesh for a flow cell biochip and its entrance tubing (h) with 30-cm length and 0.8-mm diameter.
