Extended Data Fig. 3: Assessment of height fluctuations and potential damage to the biochip by the moving PET belt.
From: Fast, cost-effective and flexible DNA sequencing by roll-to-roll fluidics
a, Experimental setup for determining vertical fluctuations of PET belt using Keyence SI-T80 by detecting air layer thickness between the PET belt and the vacuum box. b, Height fluctuations of the PET belt moving at 0.32 m/s by measurement of air layer thickness between the PET belt and the vacuum box. c–e, three sensors (A, B, Gap) show a constant 10 µm of liquid thickness before/after the biochip, and 20 µm on the biochip. f-g, side-view image of 10 µm liquid film on the PET belt by optical microscopy. h, Thickness measurement of the liquid film in the width direction using a Keyence SI-T80. i, Photograph of a DNA sequencing biochip (7×7 cm2) with a handle attachment at the top. j, k, Fluorescent images of the biochip where the gap heights to the moving PET belt are 10 μm and 0 μm, respectively. Note the scratched (red circled) regions for 0 µm.
