Extended Data Fig. 7: Evaluation of 2,4-DIH’s potential to attenuate cisplatin-induced acute kidney injury.
From: High-throughput profiling of chemical-induced gene expression across 93,644 perturbations
a, Body weight changes of mice in each group during treatment (n = 10 for each group, one-way ANOVA). b, The effects of cisplatin and 2,4-DIH on kidney coefficient. The kidney coefficient was calculated as the ratio of the kidney weight to the total body weight of KM mice (n = 10 per group, one-way ANOVA). c, Quantitative analysis of iron deposition in kidney (n = 6 per group, one-way ANOVA). The treatment dosage of 2,4-DIH is 100 mg/kg, while that of cisplatin is 25 mg/kg. d, Representative IHC staining for Nrf2 expression in kidney tissues (n = 3 for each group). Scale bar, 200 µm (upper panel) and 50 µm (lower panel). e, Quantitative analysis of Nrf2 expression in kidney tissues (n = 3 for each group, two-sided Student’s t-test). All data are denoted as mean ± SD. 2,4-DIH, 2,4-dihydroxybenzaldehyde.
