Extended Data Fig. 7: Widefield live single-molecule RNA imaging comparison of MCP variants. | Nature Methods

Extended Data Fig. 7: Widefield live single-molecule RNA imaging comparison of MCP variants.

From: RNA-stabilized coat proteins for sensitive and simultaneous imaging of distinct single mRNAs in live cells

Extended Data Fig. 7

Widefield fluorescence microscopy images of live U2OS cells transfected to express mCherry-24xMS2-pA mRNA and HA-dMCP (left), HA-NLS-tdMCP (center), or HA-tdMCP (right) fused to (a) 1xmNG or (b) 4xmNG. All images are single frames extracted from live recordings that were obtained using the same microscope settings throughout. Images are displayed with multiple brightness settings to more fully portray the relative image quality produced by each variant.

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