Extended Data Fig. 9: Subcellular distribution of LSS-mCherry-KDEL-16xMS2 mRNA movements relative to distribution of LSS-mCherry-KDEL protein expression.
Data from three different regions of live U2OS cells expressing 4xmNG-dMCP and LSS-mCherry-KDEL-16xMS2-pA RNA. 4xmNG-dMCP signal is displayed in the top row and LSS-mCherry-KDEL signal is displayed in the middle row. The bottom row depicts RNA particle traces color-coded by apparent diffusion coefficient as previously described and overlaid on top of LSS-mCherry-KDEL signal. It can be seen that most dMCP-labeled RNA particles in these cells are static, and that they tend to accumulate in regions with higher LSS-mCherry-KDEL signal.
