Extended Data Fig. 10: Subcellular distribution of TOM20-mCherry-16xMS2 mRNA movements relative to distribution of TOM20-mCherry protein expression.
Data from three different regions of live U2OS cells expressing 4xmNG-dMCP and TOM20-mCherry-16xMS2-pA RNA. 4xmNG-dMCP signal is displayed in the top row and TOM20-mCherry signal is displayed in the middle row. The bottom row depicts RNA particle traces color-coded by apparent diffusion coefficient as previously described and overlaid on top of TOM20-mCherry signal. It can be seen that dMCP-labeled RNA particles in these cells are in visibly distinct static and moving populations, with static RNA tending to concentrate near regions of high TOM20-mCherry signal and moving RNA tending to be found in regions lacking this signal.
