Extended Data Fig. 2: Extended data of dMCP with various fluorescent and bioluminescent fusion partners.
(a) Full-well views of the HEK293FT cells shown in Fig. 1f display dMCP’s sustained performance across a large population of transfected cells. Tiled fluorescence microscopy images of five dMCP fusion proteins are shown, with fluorescence emissions ranging from cyan to far-red wavelengths. The signal from mCherry or mTurq2 cotransfection markers are shown in the bottom right windows. (b) MS2-RNA stabilization of an NLuc-dMCP fusion construct. Relative NLuc fluorescence was normalized to Fluc co-transfection marker and quantified by plate reader. HEK293FT cells were co-transfected with NLuc-MCP variants and either H2B-mCherry-pA (gray circles) or H2B-mCherry-16xMS2-pA (red circles). Individual points, bars, and error bars represent the measured intensity, mean and S.D. of n = 3 independent transfections.
