Extended Data Fig. 8: POU5F1 locus in BE screen.
From: Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing
a, Intended edit to be introduced by base editing gRNA is shown at the POU5F1 locus and its impact on gene expression. ***P < 10−4, MAST with Benjamini-Hochberg correction. n = 46 cells (POU5F1:REF), 4172 cells (POU5F1:HET) and 1163 cells (POU5F1:ALT) from 1 SDR-seq experiment. P-value = 5.78x10−20 (POU5F1: HET-ALT), b, All measured variants in combination along the measured gDNA site shown for POU5F1 with their impact on gene expression. Number of cells are indicated on top. c, Comparison of variants at POU5F1 locus identified with SDR-seq to bulk amplicon sequencing.
