Fig. 2: SDR-seq scales to hundreds of targets simultaneously.
From: Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing
a, Outline of panel size testing experiments. gDNA and RNA targets are equal within panels; shared targets are indicated. b,c, Pearson correlation of detection (b) and coverage (c) of shared gDNA targets between panels. d,e, Pearson correlation of detection (d) and coverage (e) of shared genes between panels. f, Outline of chromatin sites tested. A combination of the chromatin marks detected and the relative distance to a gene define regulatory elements; PLS, promotor-like sequence; pELS, proximal enhancer-like sequence; dELS, distal enhancer-like sequence. g, Detection of different chromatin sites between panels. Size indicates the percentage of cells detected in. Color indicates read coverage. h, Outline of expression levels tested. i, Detection of genes with different expression levels between panels. Size indicates the percentage of cells detected in. Color indicates read coverage. j, Heat map of expression of all shared genes between panels; z score data are scaled by row.
