Extended Data Fig. 3: SDR-seq with separate library generation for RNA and gDNA targets.
From: Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing
a–d, Subsampled reads/cell for all gDNA (a) or RNA (c) targets or shared gDNA (b) or RNA (d) targets (d). n = 9680 cells (120 panel), 6610 cells (240 panel) and 804 cells (480 panel) from 1 independent SDR-seq experiment for each panel size testing. e, Overview of SDR-seq with separate library generation for RNA and gDNA. Distinct R2 (RNA) or R2N (gDNA) overhangs for each library. Sequencing ready libraries can be generated using specific library primers binding to R2 or R2N, respectively. f, Specificity of gDNA and RNA NGS libraries. Data from gDNA or RNA libraries was mapped to either gDNA or RNA references. Data points represent means ± SEM. n = 3 independent SDR-seq experiments.
