Extended Data Fig. 4: Metrics for target detection and coverage across differently sized target panels.
From: Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing
a, Quality metrics of panel size experiment. Color indicates fraction of gDNA targets/cell recovered. b, gDNA coverage and detection for all targets across panels tested. Dashed line indicates 80% detection. c, gDNA target detection per cell for all targets across panels tested. n = 9680 cells (120 panel), 6610 cells (240 panel) and 804 cells (480 panel) from 1 independent SDR-seq experiment for each panel size testing. d, gDNA coverage and detection for shared targets across panels tested. Dashed line indicates 80% detection. e, gDNA target detection per cell for shared targets across panels tested. n = 9680 cells (120 panel), 6610 cells (240 panel) and 804 cells (480 panel) from 1 independent SDR-seq experiment for each panel size testing. f, Quality metrics of panel size experiment. Color indicates fraction of RNA targets/cell recovered. g, RNA coverage and detection for all targets across panels tested. Dashed line indicates 80% detection. h, RNA target detection per cell for all targets across panels tested. n = 9680 cells (120 panel), 6610 cells (240 panel) and 804 cells (480 panel) from 1 independent SDR-seq experiment for each panel size testing. i, RNA coverage and detection for shared targets across panels tested. Dashed line indicates 80% detection. j, RNA target detection per cell for shared targets across panels tested. n = 9680 cells (120 panel), 6610 cells (240 panel) and 804 cells (480 panel) from 1 independent SDR-seq experiment for each panel size testing.
