Extended Data Fig. 3: Supplementary Data for Fig. 3.
a) Flow-cytometry measurements of CD71 and CD235a levels in erythroid-differentiated HSPCs on day 5, 9 and 13. b) Flow-cytometry measurements of CD71 and CD235a levels in erythroid-differentiated HSPCs (day 5), transduced with indicated sgRNAs and electroporated with Cas9 protein. c) DNA gel of TDAC-seq library amplified at the HBG1/2 locus from erythroid-differentiated HSPCs (day 5), transduced with sgRNA-68 or non-targeting sgRNA and electroporated with Cas9 protein. d) Top six abundant genotypes (middle) at the HBG1/2 locus from erythroid-differentiated HSPCs (day 5) transduced with sgRNA-68, along with their relative abundance in sequencing reads (left) and corresponding TDAC-seq signals (right) compared to the non-targeting sgRNA control. TDAC-seq signal represents the average number of DddA11 edits in a 100-bp window. e) Scatter plots showing the number of DddA11 edits in 150-bp sliding windows across the amplicon (chr11:5245964-5257447) for the top six most abundant genotypes in samples transduced with sgRNA-68. Each axis represents replicate 1 (x axis) and replicate 2 (y axis). Experiments for data in a and b were performed once. Data c-e present n = 2 replicates. See Supplementary Fig. 2 for flow gating schemes.
