Extended Data Fig. 10: GAT1 lipidation fills a potential lipid binding site by mimicking cholesterol. | Nature Methods

Extended Data Fig. 10: GAT1 lipidation fills a potential lipid binding site by mimicking cholesterol.

From: Uncovering hidden protein modifications with native top-down mass spectrometry

Extended Data Fig. 10

a, Structure of human GAT1 (PDB ID 7Y7Y) with a palmitate moiety attached to Cys493 (grey). b, Structure of porcine SERT (PDB ID 8DE4) with a bound cholesterol hemisuccinate lipid (grey). c, Sequence alignment of human GAT1 and porcine and human SERT. The lipidated cysteine in GAT1 (Cys493) is not present in either SERT, preventing lipid conjugation at this site.

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