Supplementary Figure 3: Tight junction protein levels in the hippocampus (HIPP) and prefrontal cortex (PFC) following 10-d CSDS

A) Cldn5 (*p = 0.0236) and occludin (*p = 0.0327) protein levels were significantly higher in RES mice when compared to SS, but not unstressed control mice. Occludin protein level was positively correlated with SI ratio (*p = 0.0486). No difference was observed for ZO-1 or the endothelial cell marker CD31. B) 10-day CSDS had no effect on tight junction protein levels or CD31 in the PFC. Scale bar at 200 µm. Images corresponded to flattened 1-µm-thick z-stacks at 40x magnification. Data represent mean ± SEM, number of animals (n) is indicated on graphs. One-way ANOVA followed by Bonferroni’s multiple comparison test and Pearson’s correlation for tight junction protein level vs SI ratio, *p < 0.05